. . 11. , 2007. . 93-99. - FESTUCA PRATENSIS (POACEAE) . . , . . , ( c ) . , . (, -), c . - . C , , ( ). FESTUCA PRATENSIS (POACEAE) T. S. NIKOLAEVSKAYA, O. N. LEBEDEVA. POLLEN FERTILITY IN MUTANT POPULATIONS AND PLANTS WITH SUPPRESSED CHLOROPHYLL DEFICIENCY IN In allogamous species meadow fescue the pollination system is accompanied by producing of large amount of pollen possessing ecological plasticity and genetic quality what affects the microgametes value during selection and competition ability of mature pollen. In this connection the state and the level of diversity of microgametophyte fertility were investigated in meadow fescue plants differing in genetic background (mutant populations and plants with suppressed chlorophyll deficiency) and ontogenetic age. 7 mutant populations cultivated for 5 generations and 110 revertant in chlorophyll deficiency clones cultivated for 6 vegetation periods were studied. It was shown that fertility of the male gametophyte in experimental meadow fescue plants during 5 generations as well as 6 years of life was remarkable for diversity and large varying values. The level of expression and variability of pollen fertility depended on specific genetic (genotypic features of mutant populations and revertants) and nonspecific (number of generations, age) factors. Specific adaptive state in populations was determined by natural selection, particularly by its stabilising form (based on deviation from the mean analysis in the pollen fertility trait). Stabilising selection was mostly favourable to mutant populations formed on the basis of chemical mutagens as compared with gamma-populations and revertants with lighter expression of chlorophyll deficiency phenotype (viridis and xantha) as well. ______________________________________________________________________________________ (, 1978). , (, 1995). (Auquier, 1977). , -- , - 93 . (, 1971; , 1987; Mulcahy, Mulcahy, 1987; , 1994). , (Welch, Klatt, 1971; Jones, 1976), , . , , (Frling, 1981; Grgiw, 1981; Niln et al., 1981; , 1997; Luna et al., 2001; Westgate et al., 2003; Wang et al., 2003). , ( c ) . Festuca pratensis Huds. , ( -- , -- -- NaN3) - (, , 1985). , . , , (35°C) , (Land et al., 1971). , , -, ( ., 1978; ., 1982). (- ., 1994), . (25°C) () ( ). 94 (, , 1974). 7 : V -- , VN -- -, VX -- -, X -- , XN -- -, XV -- -, A -- . : ( 4 25°), V>N -- - , X>N -- , A>V -- ( 14 ), V>V -- -, X>X -- -, >A -- -. -- W-type (N, ). , , - 7 110 (8 60×60 ). ( ) . 6 (1997-2002 .). 700 . () 1% . , - . , , . ( 2000 ) 50 . - . (, , , ) . ( ) : , -, , -, . . ( - ) () . : y=a+bx, y ­ ; ­ Y; b ­ tg , x ­ . (0,82-0,94). -, . 0,54 0,88. MS Excel 6.0. (. 1). : -- , ( ) -; -- , , , (+, +) NaN3-; +NaN3- . (2-5) , . , , : , NaN3. . , , , . , , (. 2). (W-type) -, . - VN- , XN- VX- . , , . , VN-, XV- - , VX-, X- XN- , - . 1. 1-5- + + +NaN3 NaN3 - , % 3 4 91,2±0,2*** 82,0±0,6*** 85,2±0,3** 96,1±0,2*** 92,3±0,2*** 91,2±0,2*** 69,0±0,4*** 87,5±0,2 88,0±0,8 95,3±0,6*** 94,1±0,5*** 94,4±0,6*** 88,2±1,0 90,4±0,7* 88,6±1,0 88,5±0,9 1 48,3±1,3*** 74,1±1,0*** 64,3±1,1*** 81,4±0,8*** 69,2±1,1 64,4±1,1** 76,5±0,9*** 69,4±1,1 2 75,1±0,8*** 86,1±0,5*** 79,8±0,6*** 77,2±0,7*** 48,8±0,6*** 68,4±0,81** 77,2±0,6*** 64,5±0,7 5 87,5±1,2 90,8±1,1** 91,1±0,3*** 92,7±0,7*** 88,3±1,5 89,4±0,3* 91,5±0,6*** 85,3±1,5 . * ­ p<0,05, ** ­ p<0,01, *** ­ p<0,001. 95 2. - W-type V VN VX X XN XV , %, 1997 1998 1999 2000 2001 2002 89,0±0,8 87,1±1,6 91,9±1,0 89,7±0,9 81,1±1,4*** 77,3±2,1* 90,3±0,8 87,7±1,3 89,9±0,8 88,1±1,0 90,5±0,7 90,7±0,9* 90,6±1,7 87,4±2,5 - 85,9±1,9 82,4±1,6 89,7±1,3 88,3±1,2 88,6±1,5 80,7±2,7 90,3±1,7 91,6±1,2 88,3±1,4 59,4±5,7*** 70,1±3,3*** 82,0±1,5** 79,8±2,4* 81,7±2,1 90,9±0,9 88,4±1,0 84,9±1,2 78,0±2,2 88,8±1,9 87,1±0,9 71,4±3,7*** 76,8±1,6* 86,8±0,8 88,4±1,3 90,5±1,7 88,4±1,6 91,2±1,5 86,2±1,6 . . 3: (W-type) * ­ p<0,05, ** ­ p<0,01, *** ­ p<0,001. -, () , (. 3). , -, , . - , , , . - , - , . , (- , , ) . , . , . 1-5- «» ( ) 61,08% . ( ) (. 4). ( ) . 3. - V>N X>N A>N V>V X>X A>A A , %, 1997 89,6±1,1 40,5±2,7 93,9±03 1998 72,6±2,5 69,7±2,2 83,8±1,5 1999 92,9±1,2 85,5±1,3 91,6±0,4 2000 95,2±0,7 73,4±0,9 90,9±1,4 2001 93,0±1,4 83,7±1,3 86,2±1,3 2002 94,9±0,4 82,6±1,4 91,9±0,4 94,7±0,7 90,1±0,8 63,4±5,3 74,6±3,3* 87,4±1,9 89,9±1,1 39,9±6,4 85,1±1,9 88,5±2,5 85,9±1,7 51,7±2,1 66,3±1,6*** 93,9±1,7 90,4±1,0 54,4±2,8 88,1±1,2 93,5±0,9 89,8±0,9 70,4±2,1 79,9±1,6** 97,8±0,1 90,3±0,8 64,1±3,3 77,0±2,1** 96 4. - 7 4 28 39 7 5 35 47 73,57 880,84 61,78 135,59 128,09 22,99 F (.) F (.) 1,19 2,36 14,26 2,71 , % 8,93 61,08 29,99 39,64 26,74 33,62 5,90 5,57 2,29 2,49 : F(.) ­ , F(.) ­ =0,95. , 30,92%, -- 26,15, -- 12.52% . , , ( ) . - ( ) , , (. 4). , «» , , .. , . , ( ) , ( ) () . (, 1969; , 2003). (+ -) ( , ) , , . , - -, , (., ). , - . (., ). , . , ( ), , , , -- -. -, . - , , ( ., 2002) , , . , , , . . 97 40 30 20 10 0 0 -10 -20 -30 -40 40 30 20 10 0 0 -10 -20 -30 -40 3 6 3 6 9 12 15 9 12 15 , , - . F. pratensis ­ : × ­ , ­ ­ ; ­ -. ­ - : ­ , × ­ -, ­ -, ­ -. ­ ; ­ () - () , , , , . (, -), . - 98 - . , , -, ( ). . . . . . . ., . ., . . 1978. . . 1987. // . . . 103. 2. . 298-313. . . 2003. . .: «». 431 . . ., . ., . . . 2002. Festuca pratensis (Poaceae) // . . . 87. 12. . 48-58. . . 1978. // . . . . 39. 5. . 743-751. - . . . 1994. : // . . 30. 8. . 1022­1035. ., . 1974. . : . 60 . .. 1995. // . . 6. . 76-82. . . 1997. Festuca pratensis (Poaceae) // . . . 82. 8. . 88­93. . ., . . 1985. , 1 , // . . 11. . 56-59. ., . ., - // . . . . 39. 4. . 628-632. . . 1969. . .: . 494 . Auquier P. 1977. Biologie de la reproduction dans le genre Festuca L. (Poaceae). 1. Systemes de Pollinisation // Bull. Soc. roy. bot. Belg. V. 110. P. 129­150. Frling . 1981. wrd mnitring sifi DN lsins in th gn busing sstms // nvirn. lth rst. V.37. P. 13-17. Grgiw S. . 1981. l stril mutnts indusd in . stivum ftr S trtmnt // . . . . 35. . 241-244. Jones A. G. 1976. Environmental effects on the persentage of stainble and presumed normal pollen in Aster (Compositae) // Amer. J. Bot. V. 63. P. 657-663. Land J. B., Whittington W. J., Norton G. 1971. Environment dependent chlorosis in mutant plant of Festuca pratensis Huds. // Ann. Bot. V. 35. P. 605-613. Luna S. V., Figueroa M., Baltazar M. et al. 2001. Maize pollen longevity and distance isolation requirements for effective pollen control // Crop. Sci. V. 41. P. 1551-1557. Mulcahy D. L., Mulcahy G. B. 1987. The effect of pollen comptition // Amer. Sci. V. 75. P. 44-50. Niln R. ., Rsihn J .L., rnz ., dgdn . L., linhfs . 1981. lln gnti mrkrs fr . ., . ., . . 1982. . .: . 112 . . . 1994. - // . 10. . 1423-1431. . . 1971. . // . . . . . 3. . 62­66. dttin f mutgns in thnvirnmnt // nvirn. lth. rst. V. 37. S. 19-25. Wang Z.-Yu., Ge Y., Scott M., Spangenberg G. 2004. Viability and logevity of pollen from transgenic and nontransgenic tall fescue (Festuca arundinacea) (Poaceae) plants // Amer. J. Bot. V. 91. P. 523-530. Welch J. R., Klatt A. R. 1971. Effects of temperature and photoperiod on spring wheat pollen viability // Crop. Sci. V. 11. P. 864-865. Westgate M. E., Lizano J., Batchelor W. 2003. Quantitative relationships between pollen shed density and grain yield in maize // Crop. Sci. V. 43. P. 934-942. 99